I have been trying to patch from Purkinje neurons and am having difficulty. I can seal the cells very consistently, however when I apply suction, the seal drops immediately down to less than 100 MOs nearly every time. I am not sure if it is the quality of the cells or a problem with the solutions. The internal solution has an osmolarity of 290, and the external (Tyrode's) is at 320. Any suggestions would be immensely appreciated!
Thanks so much!
Loosing seal going whole cell