lack of Spontanteous EPSC in clampex?

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frustrated student
frustrated student's picture
lack of Spontanteous EPSC in clampex?

Hi-

I have another issue...

When I am in i-clamp, I can see my activity without any issue.  when i switch to v-clamp and want to see spontaneous EPSCs and minis, I can't seem them under any setting in clampex.  When I use axoscope, it appears that i might have some sort of activity but its tough to correlate.  the best answer i have come up with is that i am close to the reversal so I'm missing it some how.  other than that, i have no idea...i am using internal and external solns from a paper that reports minis and EPSCs routinely.

vilsy
vilsy's picture
Hi,

Hi,

Have you tried looking at EPSCs at more hyperpolarised potentials? EPSCs should become bigger with more hyperpolarization making it easier for you to see them.

Cheers,
V

neuro
neuro's picture
what happened with your minis

what happened with your minis?  if you're holding your cell at -70 mV or so they should be obvious, unless your signal:noise is low.  hold your voltage at the same resting potential that you record while in current clamp.