Issues with membranes

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Moonshoon
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Issues with membranes

Hi everybody,
I have very big troubles with the membrane of neurons in slices. I have changed my glassware, the tubings, the solutions and I keep having membrane which are very stiff and thus which are not sticking to my electrodes.
Any ideas ?
Thank you

Moonshoon
Moonshoon's picture
Did it happen to one of you

Did it happen to one of you to have troublesz with his gaz (like, bad ratio of O2/CO2) ?