interactive patch-clamp tutorial

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aderoos
aderoos's picture
interactive patch-clamp tutorial

I made two interactive Java applets that I would like to share. They show how to correct for pipette capacitance, series resistance etc. Basically, you can simulate an experiment from putting the pipette in the bath, to cell-attached and whole cell.

Patch clamp tutotial I
Patch clamp tutorial II

Let me know what you think of it.

Best regards, Albert

Fraser Moss
Fraser Moss's picture
It's quite nice

It's quite nice

I noticed however that in Patch Clamp I, adjusting the Rs compensation (Pred/Corr) did not improve the speed of the voltage clamp.

aderoos
aderoos's picture
Thanks fraser for the

Thanks fraser for the feedback, I'll have a look at it. It has been 7 years since I looked at them so I have to dive in the code. Maybe a scaling factor, the dials have some effect.

Guy Sovak
Guy Sovak's picture
Thank you Albert for sharing

Thank you Albert for sharing with us the patch clamp applets.
it did not work correctly on my Safari but on the with the explorer it works OK.
I like the applet very much.
Guy

qucheng
qucheng's picture
I just want to watch

I just want to watch

Hss
Hss's picture
 ...one small question: here

 
...one small question: here I attach two figures that show two differents shapes that I get after going to whole cell; sometimes  I get one of them and sometimes the other one. I would like to know what is behind this difference.  

Thanks.

carolinvs
carolinvs's picture
Hi

Hi

I would like to have a look at the tutorials, can they still be found somewhere?

thanks in advance, Carolin

Sodium_fan
Sodium_fan's picture
Hi,

Hi,

Me too.
I would like to have a new link if you can to these tutorials.

thanks in advance,
Sodium_fan

Rajbanala
Rajbanala's picture
Dear Albert,

Dear Albert,

           I am very much interested in learning the electrophysiology techniques and desperately looking for online tutorials, and unfortunately i found about this forum and hoping that I would be able to get help through this forum, so kindly upload new tutorial links. Thanx in advance.

Best regards
Raj