How long could you perform recording experiment in case of primary cell?

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How long could you perform recording experiment in case of primary cell?

Hi, I'm performing whole cell patch recording with primary embryonic hippocampal neurons. I'm having trouble maintaining cell viability under our perfusion system. Generally, the cells don't look happy, and about two hours after starting experiment, most cells have serious problem.

So, I'd like know generally how long the cultured cells could be maintained under the bicarbonate buffered bath solution. 

Thank you.