how long can you keep whole cell?

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lazy
lazy's picture
how long can you keep whole cell?

I like to improve the whole cell recording time. If I could always keep it ong, it must be very fun to work. I understand we need patience,technical skill and good luck.
 
I cannot keep whole cell config for 30 min. Probably my average for keeping whole cell config is less than 10 min. How can I make it longer (about 30 min)? Cells are CHO. Very standard bath and pipette solutions.

And I like to know in general, how long (average) can you keep a good whole cell config? In order to improve it, what you have done?  
Thanks.

neuro
neuro's picture
i can hold onto a neuron in a

i can hold onto a neuron in a brain slice for an hour, but i've got to check the electrode position frequently to make sure that any small movement in the slice doesn't cause me to loose the cell