FORM OF EGTA IN PATCH PIPETTES

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Susan Travers
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FORM OF EGTA IN PATCH PIPETTES

Can anyone provide insight into the optimal form of EGTA to use for whole cell patch clamping from neurons? We had been using the sodium salt, but it occurred to us that this might increase the intracellular Na+ concentration to non-physiological levels; is is better to use the potassium salt or free form?  Thanks for any ideas...

The FFM
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2mM Mg-ATP is pretty standard

2mM Mg-ATP is pretty standard