Can not open in whole cell Patch Clamp on iPSC

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qlocean
qlocean's picture
Can not open in whole cell Patch Clamp on iPSC

Currently I am doing patch clamp on iPSC with whole cell mode. The problem is I can not open the patch to achieve the whole cell. It seems the membrane of iPSC is very thin and fragile, which is different from other normal cell lines. Does anyone have the experience with whole cell patch clamp on iPSC? Can you share your tips that how to open the patch by negative pressure or zap? I would be appreciate it!

Cinpy
Cinpy's picture
 Use a thinner glass eletrode

 Use a thinner glass eletrode compared to the one used on brain slice, and sub it very slightly. It has been a long time since your question was raised, but I wish that I would help : )