Analysis of spontaneous activity from a single neuron

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c.hill
c.hill's picture
Analysis of spontaneous activity from a single neuron

Hi there,

Basically, I've been recording mIPSCs and need to analyse the data. The aquisition software used was Patchmaster (for HEKA EPC10 amp).

Does anyone know of a way to analyse continuous data generated in Patchmaster?

Analysis programs that I have access to are Fitmaster and Neuroexplorer. Can anyone recommend a program?

Many thanks in advance :)

c.hill
c.hill's picture
I've managed to sort it out

I've managed to sort it out by exporting the data as .bwav files from Fitmaster and importing them into Axograph (v 4.0). From there I set up a variable amplitude template for detecting events and the software obtained the number, amplitude, half-width and inter-event interval of events. After that I could then make cumulative frequency graphs.

Philly
Philly's picture
 Try MiniAnalysis software

 Try MiniAnalysis software

you will beed to export your patchmaster recordings in a compatible format (e.g., bwav) and then use the file converter that comes with MiniAnalysis to convert the file again.

tensai
tensai's picture
c.hill,

c.hill,

can you post the setting you used for axograph (template threshold, etc)?

I can't seem to find a sweet spot that identifies the majority of peaks.

thanks,
t

c.hill
c.hill's picture
The template function I've

The template function I've been using is:

Template amplitude (pA) = -2
Template rise (ms) = 0.5
Template decay (ms) = 20
Template baseline (ms) = 10
Template length (ms) = 10

However, I have found notes from someone who once worked here  (patching cerebellar Purkinje cells) and their template function is the same apart from Rise = 1 and Decay = 7.

Does that help?