2 questions: 1) cell attached configuration; 2) inside-out configuration

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engstar
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2 questions: 1) cell attached configuration; 2) inside-out configuration

1) It is often said that in CELL-ATTACHED configuration, if a drug applied to the bath solution causes the change in the single channel current at the patch, there must be some intracellular messenger(s) that mediate the modulation.
The problem is: if the drug causes a change in the resting potential by affecting the channels in the large lower membrane(ie. the quasi-whole cell membrane), the change in resting potential may also cause the change in the single channel current at the patch. How can this case be excluded?

2) To form inside-out patch configuration after giga-seal formation, the pipette should be pulled away from the cell RAPIDLY or SLOWLY?  We saw such a difference in describing this method in different materials.