Hi, I'm Platelet Pete. I do injury research (burn, ischemia/reperfusion). I would like to find a simple straightforward method to determine pulmonary edema using Evan's blue or fluorescent albumin (low mw and high mw). What I would like is a quick and easy method whereby I inject the dye(s), perfuse to wash out the dyes in the vasculature and then snap freeze the tissue for frozen sections and fluorescence microscopy for the tags. Evan's blue fluoreses red using the FITC channel. Thanks.