osteoclast staining

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ghazaleh
ghazaleh's picture
osteoclast staining

hi
I needed to ask for a osteoclast staining protocol other than TRAP.  My samples are decalcified and paraffinized mandibular alveols.
I was wondering if there is a known osteoclastic marker that makes the western blot analysis a feasible option.
I would love to get all the suggestions and helps.
G  
 

Guy Sovak
Guy Sovak's picture
Osteoclast markers

Hi , There are some osteoclastic markers that you can use:RANK, OSCAR, CalcitoninPlease look at this paper http://www.ncbi.nlm.nih.gov/pubmed/2547591 Guy