Cell starvation

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Guy Sovak
Guy Sovak's picture
Cell starvation

Dose anyone use OptiMem for cell transient transfection.
Do you know how long I can keep BHK cells in this Medium untill changing it to DMEM:F12 supp with FCS

vanishing
vanishing's picture
hi gsovak,

hi gsovak,

according to

Storch et al., 2003 (Mutational analysis in longest known survivor of mucopolysaccharidosis type VII)

you can leave them at least for 5 hours in optimem

I guess that should be long enough for a transfection

http://www.springerlink.com/content/avr1qjkxa16mmuxh/fulltext.pdf

(BHK cells were transiently transfected with wild-type and mutant β-glucuronidase cDNAs in pcDNA3.1(+) or with vector alone by using 8 μl Lipofectamine and 2 μg plasmid DNA in a total volume of 200 μl OptiMEM in 35-mm dishes. After a 5-h incubation, medium was replaced with DMEM containing 5% FCS and penicillin/streptomycin.)

Guy Sovak
Guy Sovak's picture
Thanks Vanishing,

Thanks Vanishing,
I am doing a 12 incubation with Opitimem after transfection and it is working now.
Guy