G-CSF Bioassay Method

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Chintan Patel
Chintan Patel's picture
G-CSF Bioassay Method

Dear All,

Can anybody explain me how to conduct G-CSF Bioassay on mouse. If anyone can give me the method of this bioassay will help me a lot.

Thanx in advance

marcus muench
marcus muench's picture
Do you know what you would

Do you know what you would like your endpoint ot be?

If you inject G-CSF you will get a large increase in neutrophil production. Bone marrow cellularity will increase, but only about 1.5-fold. the number of immature and mature neutrohpils will increase dramatically. Hematopoiesis will shift to the spleen and its size (cellularity) will increase greatly. Blood levels of neutrophils will also increase greatly. All these are measurable parameters.

Chintan Patel
Chintan Patel's picture
Thanx for your reply,

Thanx for your reply, actually i wanted to know about any specific assay method, which is given by any pharmacopia for the testing of drug before puting every batch in market (a specific assay as a pharmacopial requirement)

Guy Sovak
Guy Sovak's picture
Hi,

Hi,
I think that this could help you,
http://www.usbio.net/Product.aspx?ProdSku=G8950-06A
This is an Elisa kit, I am not sure that if this is what you asked for.
I didnt use it so I dont know if it is good or not.

Chintan Patel wrote:

Dear All,

Can anybody explain me how to conduct G-CSF Bioassay on mouse. If anyone can give me the method of this bioassay will help me a lot.

Thanx in advance

Chintan Patel
Chintan Patel's picture
Thanx for your kind help

Thanx for your kind help

Arun Bhat
Arun Bhat's picture
Hi Mr Chinatan Patel,

Hi Mr Chinatan Patel,

Did you get the answer for GCSF Bioassay ? can youplease pass it on to me, i too want to do it on mouse.

Thanks
Arun
Raj Biotech ( India ) Pvt Ltd

Winner
Winner's picture
 Hi Mr. Arun,

 Hi Mr. Arun,

I am also working on G-CSF, but I work on invitro assay. Do you have any protocol for invitro bioassay of G-CSF? If you hv any protocol can please share it?

Thanks....