elisa assay without a plate reader?

3 posts / 0 new
Last post
Aussiescientist
Aussiescientist's picture
elisa assay without a plate reader?

I am working in a rather under resourced lab and don't have access to a plate reader. Has anyone tried reading an ELISA assay by transfering to microcuvettes and using a spec? If so have you had any success?

Guy Sovak
Guy Sovak's picture
Yes

Yes
I did it long long ago.
All depend how many samples do you have and more important what is the wave length you want to read and do you have the correct filter to read. One more question is the volume of your sample.
Guy

cellsleuth
cellsleuth's picture
 

 
Hi Guy
What volume do you need and what wavelengths work for this? ( in case they turn down my request for a plate reader)