DSP cross linkining

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Guy Sovak
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DSP cross linkining

Dear All,
I am trying to use DSP (Dithiobis-succinimidylpropionate) on intact cells. I am using it in 2mM final concentration for 30min RT stopping with 20mM TRIS pH7.4. Any one used a different protocol?
Guy

cheng_83
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sorry, i do not know what you

sorry, i do not know what you mean?

Guy Sovak
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Thanks for your reply,

Thanks for your reply,
Fortunately I managed to overcome the problem.
The concentration was 100 times higher than I have use at the end.
The protocol that was described in a paper was not correct.
Guy