what is the effect of Carbon loading in column if increase or decrease.

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Jaiveer
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what is the effect of Carbon loading in column if increase or decrease.

what is the effect of Carbon loading in column if increase  or decrease.

Rajiv Dua
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Dear Jaiveer...Carbon load is

Dear Jaiveer...Carbon load is the percent by weight of carbon on the stationary phase. How much carbon is chemically attached to the surface of silica .For a RP-HPLC column, more carbon load  means more retention for nonpolar compounds like proteins,,,,

with regards,
Rajiv