peak separation for chilli

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Esther Yap
Esther Yap's picture
peak separation for chilli

good day, i have problem in separating the peaks from 2 minutes to 6 minutes. There are at least 9 peaks that are combined with each other. The condition of hplc are as follows :Isocratic.Mobile phase : (A) Acetonitrile - 41%  (b) 0.5% formic acid (59%)Flow : 1 min/ml ; Injection : 20 microlit; Temperature : 30 degreesRetention time : 40 minsSolvent extracts : methanolColumn : C18 (250mm x 4.0mm I.D., 5 micronmeter diameter)I have run the standard and it came out nicely. Please help...