During one of the HPLC studies I have encountered a problem. I should prepare a 50mM phosphate buffer (pH=3,2) consiting of Na2HPO4·12H2O and NaH2PO4·2H2O. However I am unable to find any calculator to make it. Additionally as far as I know this buffer have reasonable capacity between pH=6 and pH=8.
Could you provide me with the input how to prepare such buffer