HPLC PROBLEM

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divyanshu
divyanshu's picture
HPLC PROBLEM

Sir im doing the related substances of paliperidone but there an impuriy peak of N -Oxime comes at RRT1.05 but if we use the new inertial ods 3-v column then this peak apper but when we use column which has injected more than 200 injestion then this peaks does not come .Give me the appropriate reason

Rajiv Dua
Rajiv Dua's picture
Dear Divyanshu....the

Dear Divyanshu....the efficacy of ur column after 200 injection will not be same as it was new....the limit LOD and LOQ value after 200 inj will change....

with regards,
Rajiv