How to do quantitativee estimation of omega 3 fatty acid

2 posts / 0 new
Last post
martingill
martingill's picture
How to do quantitativee estimation of omega 3 fatty acid

Hello,
              I want to know how one can do How to do quantitative estimation of   omega 3 fatty acid and lutein in egg and what kind of equipment we neeed.

LTD113
LTD113's picture
A very good, quantitative

A very good, quantitative method for the omega 3's is to run a "FAME" analysis.  I suggest AOCS method 1h-05.  You will need GC-FID.

For lutein, you will want to do an extraction procedure, and then analyze by LC-UV, or, better would be LC-MS.

Or you can send to an outside lab.