VOA chromatography and response issue

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Noel Blakley
Noel Blakley's picture
VOA chromatography and response issue
  • Hello,
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  • First, the peak is chromatographing unsymmetrical. Second, if you break the peak down in to two sections and get the area response, A + B doesn't = C. When quantitating the unsymmetrical part of the peak i get a response of 8800 and when i quantitate the "normal looking" part of the peak I get 312000. But when I quant the two parts together the response is 510000. I have changed the entire P&T (including transfer lines), column, inlet liners, adjusted split ratios from 20:1 to 40:1 and reinstalled software. I believe this has to do with the CO2 peak, since that peak is chromatographing strangely also. When I direct inject I don't have the problem with DCDFM but when the air comes out after the gaseous compounds, while much smaller than in a normal purged standard, it gives a unsymmetrical peak. The issue is rather difficult to explain but i would be more than happy to email a file of the standard that i ran today if anyone would like to see.
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  • Any help will be apprecited.
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  • I didn't find the right solution from the Internet.
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  • References:
  • https://bit.ly/2JqUlxR
  • Teaser production studio
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  • Thank you.