Quantitative test method of Vitamin B12

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nelunga_sonali
nelunga_sonali's picture
Quantitative test method of Vitamin B12

Hi everybody,

I need to find a more convenient and cost effective test method to analyze vitamin B12 quantitatively in a vitamin syrup.
 
Hope you will help me out.

Lysander
Lysander's picture
Hello there

Hello there

You can do this in number of ways
Every vendor has its own catalog and their sample chromatogarms- You can follow those procedures and you can achieve a successfull result.
 
I have used Discovery C18 from Sigma  15cm x 4.6mm ID x 5 um particle
and for mobile phase  A:50mM KH2 PO4
                                         B: MeOH
Flow Rate : 1 ml/min Gradient
Pressure should be reading about 1400- 1500 psi
Set the detector on UV= 220 nm
with injection size of 15 ul-

The peak of B12 should be coming out around 11 minutes
Hope this helps