I had a new Shimadzu LC2010 HPLC system recently installed. I encountered a problem of negative baseline spike at 2.7 min and a positive spike at 4.7 minutes, which is coming in all the injections, I changed a no. of mobile phaseas and the columns as well. The peak intensity is max at 200-230nm after which is goes on reducing, frequent flushing of the system also does not solve it. can any one please explaine that why these spikes are appearing and how to get rid of it?
Negative baseline spike in HPLC