Multivitamin Analysis by UPLC

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bhushanp85
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Multivitamin Analysis by UPLC

Hi freinds,

I hv started Method development for samples of All vitamins ie B complex, C, D, A, K.

I have found one method using Trifluoroacetic acid in water n ACN in gradient mode.

the thing is I have column in pH range of 2 to 7. n method mentioned above shows pH 1.4.

I tried with OPA in DW at pH 2.7 n at 3.4 n got some resolution using methanol. but consistingly changing gradient profile Im not abl to resolve them. wht other mobile phases n organic modifiers I may try to resolve them?

Also method time I want to reduce as it has to be less than conventional HPLC.

Can you help me out??