HPLC Passivation

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deleod01
deleod01's picture
HPLC Passivation

We have perfomed a passivation procedure of HPLCs in our lab using 37% Nitric acid but in some cases we keep detecting an small peak after process was finished.

Any ideas from where this peak could come from?

Have anybody had similar experience? Thanks!!!

Dr. Analytical
Dr. Analytical's picture
Passivation with nitric acid

Passivation with nitric acid will "clean" stainless steel to remove corrosion and other "active sites" on the surfaces.  These active sites will usually cause peak shape problems like tailing. but they will not produce extra peaks. 
Your extra peak not caused by active sites.  It may be a contaminant in a solvent or sample.  Please provide more information about your system and experiments, and then maybe we may have other suggestions.