I have a HPLC column with silica packings that are limited to working between pH 2 and pH 7.
We want to determine some substances in our reactions. All reactions are done in organic phase (acetone or acetonitril). Now how can we check pH for this organic phase? And how can decrease or increase it? As you know pH meter is not useful for this system!
Any partial solution to protect our column? (We don’t want change the column! I know some column is wider pH range)
HPLC column & limited pH