Gredient elution Vs Plate numbers

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Ramachandra
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Gredient elution Vs Plate numbers

Hi all 
       This is ramachandra. I have a doubt on plate numbers in gredient elution. Can we calculate plates numbers of a peaks in gredient elution mode? If  yes, plese suggest me the sutable formulae. if available, mention the references or literature.

Dr. Analytical
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Yes, you can calculate plate

Yes, you can calculate plate numbers in gradient elution, but since the peak width does not increase, the numbers will be larger as the retention increases.  Therefore, plate numbers are usually not calculated for gradients.

You could compare plate counts for different injections, but only if you use the same peak at the same retention time.  However, remember that you do this only to compare the injections, so that you can detect a change.  The actual value of the plate number will have no meaning.

Ramachandra
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Thank you Dr. Analytical for

Thank you Dr. Analytical for your reply.