Gel filtration vs ion - exchange for removal of endogenous glucosinolates

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Robin Bingham
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Gel filtration vs ion - exchange for removal of endogenous glucosinolates

 I am working on measuring myrosinase activity/levels in Thlaspi arvense using photometric quantification of the release of glucose with colorimetric indicators. The two primary references I am using on which to base my protocol differ in the type of column used, one uses DEAE sephadex G-25 and the other uses DEAE sephadex A-25. All other work with which I'm familiar uses A-25 to bind the endogenous glucosinolates for removal or isolation. I am trouble-shooting my methods because I'm not detecting any myrosinase activity yet. Up to now I've been using the Sephadex A-25. Should I try the G-25?? From my perspective that doesn't seem like it would work, but I'm considering all options to isolate myrosinase and remove the endogenous glucosinolates so that they don't interfere with quantifying myrosinase.