Formic/acetic acid by HPLC

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aliciaA
aliciaA's picture
Formic/acetic acid by HPLC

I am trying to quantitate formic acid and acetic acid by HPLC/UV and I am seeing inconsistant responses from my standards. I'm using 210nm on the detector, but I've also seen 220nm used. Wouls this work better?

I am working with ppm levels and keeping the standards at 2-8C, so I don't think that volitaization is a problem.

Any ideas?

Thanks!

Guest (not verified)
Guest's picture
What kind of column/mobile

What kind of column/mobile phase are you using?

You might find these links useful:

For reversed phase columns, Hamilton has these application notes
http://www.hamiltoncomp.com/cgi-shl/proc.exe/hcoc?applist2212

also Alltech makes a column specially for organic acid separations
http://www.alltechweb.com/productinfo/prevail/prevail.asp

ptekale
ptekale's picture
DEAR FRIEND

DEAR FRIEND
your proble is very familier. you can carry out the analysis of acetic acid and formic acid by GC using DB-624 COLUMN. injecting a injection volume of 2 ul.
work in this direction you will be definitely successful.

HPLC AT 210 nad 220 will be definitly give you more inconsistancy.

best of luck

dhruvil
dhruvil's picture
ptekale wrote:DEAR FRIEND

ptekale wrote:

DEAR FRIEND
your proble is very familier. you can carry out the analysis of acetic acid and formic acid by GC using DB-624 COLUMN. injecting a injection volume of 2 ul.
work in this direction you will be definitely successful.

HPLC AT 210 nad 220 will be definitly give you more inconsistancy.

best of luck

my request is to "ptekale".
I m working with a pharma company(in R & D) and i m also facing the same problem with the quantification of acetic acid and formic acid. i have tried a lot for developing this method on GC but i failed to do so.

Can u please guide me bout he procedure and the analytical conditions for Gas chromatography that i need to maintain for the quantification of this molecules.

thx
regards,
Dhruvil

naveen.nn
naveen.nn's picture
Dear friend,

Dear friend,

           Try it out by Gas chrmatography in CB05 columns. Instead of direct injection, dilute with some solvents like Methylene dichloride.

Best regards
NAVEEN.N.N.

Santosh@India
Santosh@India's picture
Please test the compatability

Please test the compatability of your HPLC column before injecting high acidic analyte , strong acidic compound results in to column oozing => results in to inconsistant responce. In your case this may be the reason.Use the column able to withstand such low pH.

gradlab
gradlab's picture
@ ptekale:  I know this is

@ ptekale:  I know this is late but I have a question for you.  I am trying to quantitate formic acid using a DB-624 column.  I have been unable to find it.  I have tried using a TCD & a FID with little success.  Could you please tell me what detector and oven tem you used?  Thanks so much!