formic acid detection by hplc?

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eooxx
eooxx's picture
formic acid detection by hplc?

 hi, in my lab we have a sample that we suspect has formic acid as a side product.

we're primarily looking for a way to detect, then maybe quantitate later if we do detect some.

i need details on what kind of column, mobile phase, flowrate, UV detection wavelength etc for hplc. 

thanks

Dr. Analytical
Dr. Analytical's picture
Formic acid is very soluble

Formic acid is very soluble in water, and so it will be difficult to retain it on most reversed phase columns.  You could try a "polar embedded phase" and a slightly acidic mobile phase (0.05 - 0.1% phosphoric acid).  These columns are more retentive than regular RP phases.  Typical examples are: Bonus RP (Agilent), Ultra II IBD (Restek), and RP Amide (Supelco).

Detection will be difficult with an absorbance detector - try 210 nm, but don't expect high sensitivity.  Refractice index might also be an option.