determine the concentration

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arman azadi
arman azadi's picture
determine the concentration

Dear colleagues,I'm working with RP-HPLC for the first time so I have lots of questions. I injected a sample of waste water containing nitate with known concentratition and after that, I Injected another sample which was encountered with an absorbance to remove nitrate. I obtained the peaks but I want to know the nitrate concentraion in my absorbed sample. How can I calculate that?