I'm currently trying to run peptide samples containing detergent (1%) and some hydrophobic substance in a GFC system. I want to see the separation of peaks and difference in retention times of different combination of samples. Probably will use PBS or saline as mobile phase.
I'd like to know whether having a detergent in the sample will affect the run?
is having the hydrophobic substance will affect the quality of column (it will stick to it?). i'm using superdex.
The hydrophobic substance was only soluble in a certain buffer with detergent added to it. so if i inject it in a system where only PBS or saline is present, will it then precipitate out of solution? if so, is there any suggestion as to how to do it? if using detergent in the mobile phase, the back pressure might be too high.