I did extraction of plant material with n hexane, and I collected 4 fractions into separate tubes.
Then I measured them on HPLC resulting in:
1 fraction (1 big peak of CBDA asit supossed to be)
2 fraction (shift of CBDA peak (1min to early, peak is much thinner and smaller) AND another much smaller peak was visible just next to it.
3 fraction (the same as 2nd but both peaks are smaller)
4 fraction (the same as 2nd and 3rd but both peaks are smaller)
Anybody can help and tell me what happend with CBDA in 2nd 3rd 4th fraction? For sure it's some kind of interaction of CBDA with n-hexane but I could not find any data about it in literatue.