Best TFA source?

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bwbrian
bwbrian's picture
Best TFA source?

Who sells the best TFA for use at 0.1% in HPLC solvents? I've had problems with consistency in my peptide separations, and would like to ensure that the TFA is not a question/issue in the future.

R Bishop
R Bishop's picture
We generallly use Fluka,

We generallly use Fluka, which you can buy through Sigma. But, here is an explanation of why you might be having issues.

http://www.westernanalytical.com/vydac/whyTFA.htm

RB

bwbrian
bwbrian's picture
I've solved the problem by

I've solved the problem by switching to Pierce TFA in 1 ml ampules.
http://www.piercenet.com/Products/Browse.cfm?fldID=02040623
I just add an ampule to a 1 L bottle of HPLC-grade water. I use their formic acid in amps as well.