Need help with antibody concentration/dilution calculation

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ihcgal
ihcgal's picture
Need help with antibody concentration/dilution calculation

 Hello,

I feel embarrassed to ask this , but I have to admit mathematics is my weakness. I need help with calculating for antibody concentration and dilutions. 

I have an IgG (200 microgram/0.5ml) that I need to match its concentration with 2 other ab's: (ab "A" 21 microgram/ml, and ab "B" 1000 microgram/ml) when I make serial dilutions of 1/50, 1/250 and 1/1000.

Thank you so much in advance for your help. 

g a
g a's picture
 HI

 HI

You can apply simple equations M1V1=M2V2
since MW of Ab corresponds to Molarity as well you can compare 2 different ab. For e.g Keep a fixed volume of 1 ml for all yr calculations. Now

for IgG solution you already have, 200mkg/0.5ml or 400mkg /ml, ab 2, can be matched by equation as

M1V1=M2V2 where,
M1= 400
V1= 1000
M2=1000
V2= to be calculated
 400x 1000= 1000x V2
V2= 400
or you require 400microlitres of Ab2 in a total volume of 1ml, to have th concentration equal.

ihcgal
ihcgal's picture
 Thank you very much for your

 Thank you very much for your kind and informative help!