Aldehyde analysis by HPLC method

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sukhan3
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Aldehyde analysis by HPLC method

Dear Sir

am a Ph.D scholar, working on Aldehyde determination by HPLC, I cant find any apropiate method for the same. if you can help me.
Regards

g a
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Hi Sukhan3

Hi Sukhan3

I could not locate any article for direct analysis of aldehydes but this one is quite closer as it uses the 2, 4 DNP derivatives of aldehydes.

check the following link.
http://pubs.acs.org/doi/abs/10.1021/ac980699f

Quantitative RP-HPLC Determination of Some Aldehydes and Hydroxyaldehydes as Their 2,4-Dinitrophenylhydrazone Derivatives

Dr. Analytical
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EPA Method 8315 provides a

EPA Method 8315 provides a method for determination of aldehydes.  You can search for the details on the EPA web site.  I developed the original version for formaldehyde.

Depending on your equipment, you may be able to do the derivatization on-line, using the autosampler, but that technique has not been published.

masoomeh
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 hiiiiiiiiiiiiiiiii

 hiiiiiiiiiiiiiiiii
determination of aldehyde in seawaters by uv is posible?

Dr. Analytical
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With derivatization, yes,

With derivatization, yes, aldehydes can be quantified in any water sample. 

masoomeh
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tanks..................

tanks..................
but determination of aldehyde by uv-visible is correct?
apropaite method?

Dr. Analytical
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You will use UV-Visible

You will use UV-Visible detector with a liquid chromatography (HPLC) system.  Then you can separate the individual compounds and compare to standards.  If you use only UV-Visible spectrometer, with no chromatography, then you would only measure total aldehydes and ketones, and maybe other interferences.