Topic Name

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 24 August 2010

Ekaterina, Vorotnikova , Donna, McIntosh , Abiche, Dewilde , Jianping, Zhang , Janet E., Reing , ...

Most adult mammals heal without restorative replacement of lost tissue and instead form scar tissue at an injury site. One exception is the adult MRL/MpJ mouse that can regenerate ear and cardiac tissue after wounding with little evidence of scar tissue formation. Following production of a MRL mouse ear hole, 2mm in diameter, a structure rapidly forms at the injury site that resembles the amphibian blastema at a limb amputation site during limb regeneration. We have isolated MRL blastemal cells (MRL-B) from this structure and adapted them to culture. We demonstrate by RT-PCR that even after continuous culturing of these...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 22 August 2010

Zoher, Kapacee , Ching-Yan Chloé, Yeung , Yinhui, Lu , David, Crabtree , David F., Holmes , ...

Tendon-like tissue generated from stem cells in vitro has the potential to replace tendons and ligaments lost through injury and disease. However, thus far, no information has been available on the mechanism of tendon formation in vitro and how to accelerate the process. We show here that human mesenchymal stem cells (MSCs) and bone marrow-derived mononuclear cells (BM-MNCs) can generate tendon-like tissue in 7days mediated by transforming growth factor (TGF) ß3. MSCs cultured in fixed-length fibrin gels spontaneously synthesized narrow-diameter collagen fibrils and exhibited fibripositors (actin-rich, collagen fibril-containing plasma membrane protrusions) identical to those that occur in embryonic tendon. In...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 22 August 2010

Nicholas S., Kalson , David F., Holmes , Zoher, Kapacee , Iker, Otermin , Yinhui, Liu , ...

Tendons attach muscles to bone and thereby transmit tensile forces during joint movement. However, a detailed understanding of the mechanisms that establish the mechanical properties of tendon has remained elusive because of the practical difficulties of studying tissue mechanics in vivo. Here we have performed a study of tendon-like constructs made by culturing embryonic tendon cells in fixed-length fibrin gels. The constructs display mechanical properties (toe-linear-fail stress-strain curve, stiffness, ultimate tensile strength, and failure strain) as well as collagen fibril volume fraction and extracellular matrix (ECM)/cell ratio that are statistically similar to those of embryonic chick metatarsal tendons. The development...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 20 August 2010

Debora, Gerlach , Tim, Kaminski , Fabián, Pérez-Willard , Gregor, Kirfel , Volkmar, Gieselmann , ...

The extracellular matrix of the brain is a highly organized hyaluronan-based supramolecular assembly that is involved in neuronal pathfinding, cell migration, synaptogenesis and neuronal plasticity. Here, we analyze the structure of the hyaluronan-rich pericellular matrix of an oligodendroglial precursor cell line using helium ion beam scanning microscopy at a subnanometer resolution. We find that thin nanofibers are the ultimate building elements of this oligodendroglial pericellular matrix. These structures may participate in the regulation of oligodendroglial maturation and motility.

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 18 August 2010

Helge, Wiig , Doruk, Keskin , Raghu, Kalluri

The lymphatic system is important for body fluid balance as well as immunological surveillance. Due to the identification of new molecular markers during the last decade, there has been a recent dramatic increase in our knowledge on the molecular mechanisms involved in lymphatic vessel growth (lymphangiogenesis) and lymphatic function. Here we review data showing that although it is often overlooked, the extracellular matrix plays an important role in the generation of new lymphatic vessels as a response to physiological and pathological stimuli. Extracellular matrix-lymphatic interactions as well as biophysical characteristics of the stroma have consequences for tumor formation, growth and...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 11 August 2010

Lauren, Card , Nikki, Henderson , Yuhua, Zhang , Paul, Bornstein , Amy D., Bradshaw

The sequence encoding the N-propeptide of collagen I is characterized by significant conservation of amino acids across species; however, the function of the N-propeptide remains poorly defined. Studies in vitro have suggested that one activity of this propeptide might be to act as a feedback inhibitor of collagen I synthesis. To determine whether the N-propeptide contributed to decreased collagen content in SPARC-null mice, mice carrying a deletion of exon 2, which encodes the globular domain of the N-propeptide of collagen I, were crossed to SPARC-null animals. Mice lacking SPARC and expressing collagen I without the globular domain of the N-propeptide...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 10 August 2010

Wing S., To , Kim S., Midwood

The three-dimensional organization of the ubiquitous extracellular matrix glycoprotein fibronectin regulates cell fate and morphogenesis during development; in particular tubule formation that constitutes the vasculature, lung and kidney. Tenascin-C is a matrix protein with a restricted expression pattern; it is specifically up-regulated at sites of fibronectin fibril assembly during development and in remodeling adult tissues. Here we demonstrate that specific domains of tenascin-C inhibit fibronectin matrix assembly whereas full-length tenascin-C does not. These domains act via distinct mechanisms: TNfn1-8 blocks fibrillogenesis by binding to fibronectin fibrils and preventing intermolecular fibronectin interactions whilst FBG acts independently of binding to fibronectin and...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 3 August 2010

Trushar R., Patel , Gordon A., Morris , Danicla, Zwolanek , Douglas R., Keene , Jianhua, Li , ...

Laminins are multidomain glycoproteins that play important roles in development and maintenance of the extracellular matrix via their numerous interactions with other proteins. Several receptors for the laminin short arms revealed their importance in network formation and intercellular signaling. However, both the detailed structure of the laminin ?-1 short arm and its organization within the complexes is poorly understood due to the complexity of the molecule and the lack of a high-resolution structure. The presented data provide the first subatomic resolution structure for the laminin ?-1 short arm in solution. This was achieved using an integrated approach that combined a...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 3 August 2010

Karl, Reisig , Alisa Morss, Clyne

Fibroblast growth factor-2 (FGF2) is produced and released by endothelial cells and binds to heparan sulfate proteoglycans in the endothelial basement membrane (BM), an important FGF2 storage reservoir. Experimental and computational models of FGF2 binding kinetics to both cells and BM under static conditions are well established in the literature but remain largely unexplored under flow. We now examine BM-FGF2 binding kinetics in fluid flow conditions. We hypothesized that FGF2 binding to the endothelial BM would decrease as fluid shear stress increased. To investigate this, BM-FGF2 equilibrium, associative, and dissociative binding were measured at various shear stresses. Surprisingly, FGF2 binding...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 26 July 2010

Seong-Hyun, Kim , Hae Young, Choi , Ju-Hoon, So , Cheol-Hee, Kim , Shiu-Ying, Ho , ...

The human COL17A1 gene encodes type XVII collagen (also known as the 180-kDa bullous pemphigoid antigen), an integral component of hemidesmosomes, attachment complexes providing integrity to the dermal-epidermal junction. Zebrafish, a facile model system to study skin development, displays fully developed hemidesmosomes at approximately 5days post-fertilization (dpf). We have identified two COL17A1 orthologues in the zebrafish genome, col17a1a and col17a1b, which are expressed in the skin and the neural system, respectively. The proteins coded by these genes have structural module organizations homologous to the human type XVII collagen. “Knock-down” of the expression of col17a1a with a specific morpholino targeting the...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 21 July 2010

Dario, Fortunati , Sjur, Reppe , Åse-Karine, Fjeldheim , Marit, Nielsen , Vigdis T., Gautvik , ...

Periostin is a 90kDa secreted protein, originally identified in murine osteoblast-like cells, with a distribution restricted to collagen-rich tissues and certain tumors. In this paper, we first analyzed the expression of periostin mRNA and protein in human fetal osteoblasts (hFOB) and human osteosarcoma (hOS) cell lines by RT real-time PCR and Western blot, respectively. The hFOB 1.19 and three hOS (MHM, KPDXM and Eggen) showed highly variable periostin mRNA levels and protein. Second, we showed that the expression of periostin mRNA was inversely related to the cells’ abilities to differentiate and mineralize. Then, we investigated the regulation of periostin mRNA...

Publication year: 2010
Source: Matrix Biology, Volume 29, Issue 5, June 2010, Page 439

[No author name available]

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 7 July 2010

Neil A., Turner , Philip, Warburton , David J., O’Regan , Stephen G., Ball , Karen E., Porter

The proinflammatory cytokine interleukin-1 (IL-1) elicits catabolic effects on the myocardial extracellular matrix (ECM) early after myocardial infarction but there is little understanding of its direct effects on cardiac myofibroblasts (CMF), or the role of p38 mitogen-activated protein kinase (MAPK). We used a focused RT-PCR microarray to investigate the effects of IL-1a on expression of 41 ECM genes in CMF cultured from different patients, and explored regulation by p38 MAPK.IL-1a (10ng/ml, 6h) had minimal effect on mRNA expression of structural ECM proteins, including collagens, laminins, fibronectin and vitronectin. However, it induced marked increases in expression of specific ECM proteases, including...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 6 July 2010

Bettina A., Gentry , J. Andries, Ferreira , Amanda J., McCambridge , Marybeth, Brown , Charlotte L., Phillips

Exercise intolerance, muscle fatigue and weakness are often-reported, little-investigated concerns of patients with osteogenesis imperfecta (OI). OI is a heritable connective tissue disorder hallmarked by bone fragility resulting primarily from dominant mutations in the proa1(I) or proa2(I) collagen genes and the recently discovered recessive mutations in post-translational modifying proteins of type I collagen. In this study we examined the soleus (S), plantaris (P), gastrocnemius (G), tibialis anterior (TA) and quadriceps (Q) muscles of mice expressing mild (+/oim) and moderately severe (oim/oim) OI for evidence of inherent muscle pathology. In particular, muscle weight, fiber cross-sectional area (CSA), fiber type, fiber histomorphology,...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 28 June 2010

Qirui, Hu , Adrian, Shifren , Carla, Sens , Jiwon, Choi , Zoltan, Szabo , ...

Heterozygous elastin gene mutations cause autosomal dominant cutis laxa associated with emphysema and aortic aneurysms. To investigate the molecular mechanisms leading to cutis laxa in vivo, we generated transgenic mice by pronuclear injection of minigenes encoding normal human tropoelastin (WT) or tropoelastin with a cutis laxa mutation (CL). Three independent founder lines of CL mice showed emphysematous pulmonary airspace enlargement. No consistent dermatological or cardiovascular pathologies were observed. One CL and one WT line were selected for detailed studies. Both mutant and control transgenic animals showed elastin deposition into pulmonary elastic fibers, indicated by increased desmosine levels in the lung...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 25 June 2010

Qianli, Yu , Randy, Vazquez , Sherma, Zabadi , Ronald R., Watson , Douglas F., Larson

Aberrant concentrations of cardiac extracellular matrix (ECM) fibrillar collagen crosslinking have been proposed to be an underlying cause of cardiac diastolic dysfunction however the role of the adaptive immune system in this process has yet to be investigated. Fibrillar collagen crosslinking is a product of the enzymatic activities of lysyl oxidase (LOX and LOXL-3) released by the cardiac fibroblast and possibly cardiac myocytes. Our hypothesis is that stimulation of the TH1 lymphocytes activates lysyl oxidase mediated ECM crosslinking and thereby alter left ventricular function. Three-month old C57BL/J female mice were treated with selective TH1 lymphocyte inducers - T-cell receptor Vß...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 23 June 2010

Henry J., Greyner , Tomasz, Wiraszka , Li-Shu, Zhang , W. Matthew, Petroll , Mark E., Mummert

Hyaluronan (HA) is a glycosaminoglycan composed of N-acetylglucosamine and glucuronic acid subunits. Endocytosis is thought to play an essential role in the catabolism of HA due to the intracellular compartmentalization of the HA degrading hyaluronidase enzymes. Previous investigations have shown that keratinocytes, chondrocytes and breast tumor cell lines endocytose HA via the cell surface glycoprotein, CD44. However, other cell types endocytose HA using a CD44-independent mechanism that remains to be defined. The purpose of this study was to investigate HA endocytosis in B16-F10 melanoma cells. We found that B16-F10 melanoma cells expressed CD44 on their surfaces. Unexpectedly, CD44 did not...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 21 June 2010

J.K.F., Wong , S., Al-Youha , K.E., Kadler , M.W.J., Ferguson , D.A., McGrouther

Trauma by suturing tendon form areas devoid of cells termed “acellular zones” in the matrix. This study aimed to characterise the cellular insult of suturing and acellular zone formation in mouse tendon. Acellular zone formation was evaluated using single grasping sutures placed using flexor tendons with time lapse cell viability imaging for a period of 12 hours. Both tension and injury were required to induce cell death and cell movement in the formation of the acellular zone. DNA fragmentation studies and transmission electron microscopy indicated that cells necrosed.Parallel in vivo studies showed that cell-to-cell contacts were disrupted following grasping by...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 9 June 2010

Zhuo, Xu , Naoki, Ichikawa , Keisuke, Kosaki , Yoshihiko, Yamada , Takako, Sasaki , ...

Perlecan is a component of the basement membrane that surrounds skeletal muscle. The aim of the present study is to identify the role of perlecan in skeletal muscle hypertrophy and myostatin signaling, with and without mechanical stress, using a mouse model (Hspg2-/--Tg) deficient in skeletal muscle perlecan. We found that myosin heavy chain (MHC) type IIb fibers in the tibialis anterior (TA) muscle of Hspg2-/--Tg mice had a significantly increased fiber cross-sectional area (CSA) compared to control (WT-Tg) mice. Hspg2-/--Tg mice also had an increased number of type IIx fibers in the TA muscle. Myostatin and its type I receptor...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 1 June 2010

Allison, Chatel , Rick, Hemming , Judith, Hobert , Marvin R., Natowicz , Barbara, Triggs-Raine , ...

Mammalian hyaluronidases degrade hyaluronan and some structurally related glycosaminoglycans. We generated a deletion mutant in the C. elegans orthologue of mammalian hyaluronidase, hya-1. Mutant animals are viable and grossly normal, but exhibit defects in vulvalmorphogenesis and egg-laying and showed increased staining with alcian blue, consistent with an accumulation of glycosaminoglycan. A hya-1::GFP reporter was expressed in a restricted pattern in somatic tissues of the animal with strongest expression in the intestine, the PLM sensory neurons and the vulva. Total protein extracts from wild-type animals exhibited chondroitin-degrading but not hyaluronan-degrading activity. Chondroitinase activities were observed at both neutral and acidic pH...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 31 May 2010

Hailu S., Shitaye , Shawn P., Terkhorn , Jason A., Combs , Kurt D., Hankenson

The matricellular protein thrombospondin-2 (TSP2) inhibits proliferation and enhances osteoblastogenesis of multipotent mesenchymal progenitor cells (MPC). Osteoblastogenesis and adipogenesis are reciprocally regulated, thus we hypothesized that TSP2 could be an inhibitor of adipogenesis. TSP2 gene expression is up-regulated during MPC osteoblast differentiation and down-regulated during adipogenic differentiation through a cAMP-PKA pathway, relative to control cells. Next, the importance of TSP2 in adipogenesis was studied by comparing gene-targeted knockout mice that lack TSP2 protein (TSP2-null) and control wild-type mice. TSP2-null marrow-derived MPC show 25% increased adipocytes. Similarly, TSP2-null adipose-derived MPC show increased adipocytes (25-50%) and proliferation (2-fold) relative to wild-type cells....

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 24 May 2010

LaTia, Etheredge , Bradley P., Kane , Nikola, Valdov , Sheila, Adams , David E., Birk , ...

We previously showed an agarose overlay on keratocytes cultured in media containing pharmacological levels of insulin enhanced collagen processing and collagen fibril formation. In this study, we compared collagen processing by keratocytes cultured in media containing physiological levels of IGF-I, TGF-ß, FGF-2, and PDGF in standard and in agarose overlay cultures. Pepsin digestion/SDS PAGE was used to determine the levels of procollagen secreted into the media and the collagen content of the ECM associated with the cell layer. Distribution of collagen type I and fibronectin in the ECM of the agarose cultures was determined by immunoflorescence. Collagen fibril and keratocyte...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 23 May 2010

Takako, Sasaki , Junichi, Takagi , Camilla, Giudici , Yoshihiko, Yamada , Eri, Arikawa-Hirasawa , ...

Laminin-121, previously referred as to laminin-3, was expressed recombinantly in human embryonic kidney (HEK) 293 cells by triple transfection of full-length cDNAs encoding mouse laminin a1, ß2 and ?1 chains. The recombinant laminin-121 was purified using Heparin-Sepharose followed by molecular sieve chromatography and shown to be correctly folded by electron microscopy and circular dichroism (CD). The CD spectra of recombinant laminin-121 were very similar to those of laminin-111 isolated from Engelbreth-Holm Swarm tumor (EHS-laminin) but its Tm value was smaller than EHS-laminin and recombinant lamnin-111 suggesting that the replacement of the ß chain reduced the stability of the coiled-coil structure...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 12 May 2010

Matt J., Barter , Leon, Pybus , Gary J., Litherland , Andrew D., Rowan , Ian M., Clark , ...

Histone deacetylases (HDACs) regulate the acetylation of histones in the control of gene expression. Many non-histone proteins are also targeted for acetylation, including TGF-ß signalling pathway components such as Smad2, Smad3 and Smad7. Our studies in mouse C3H10T1/2 fibroblasts suggested that a number of TGF-ß-induced genes that regulate matrix turnover are selectively regulated by HDACs. Blockade of HDAC activity with trichostatin A (TSA) abrogated the induction of a disintegrin and metalloproteinase 12 (Adam12) and tissue inhibitor of metalloproteinases-1 (Timp-1) genes by TGF-ß, whereas plasminogen activator inhibitor-1 (Pai-1) expression was unaffected. Analysis of the activation of cell signalling pathways demonstrated that...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 12 May 2010

Matt J., Barter , Leon, Pybus , Gary J., Litherland , Andrew D., Rowan , Ian M., Clark , ...

Histone deacetylases (HDACs) regulate the acetylation of histones in the control of gene expression. Many non-histone proteins are also targeted for acetylation, including TGF-ß signalling pathway components such as Smad2, Smad3 and Smad7. Our studies in mouse C3H10T1/2 fibroblasts suggested that a number of TGF-ß-induced genes that regulate matrix turnover are selectively regulated by HDACs. Blockade of HDAC activity with trichostatin A (TSA) abrogated the induction of a disintegrin and metalloproteinase 12 (Adam12) and tissue inhibitor of metalloproteinases-1 (Timp-1) genes by TGF-ß, whereas plasminogen activator inhibitor-1 (Pai-1) expression was unaffected. Analysis of the activation of cell signalling pathways demonstrated that...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 11 May 2010

Benedetta, Gualeni , Marcella, Facchini , Fabio, De Leonardis , Ruggero, Tenni , Giuseppe, Cetta , ...

Mutations in the sulfate transporter gene, SCL26A2, lead to cartilage proteoglycan undersulfation resulting in chondrodysplasia in humans; the phenotype is mirrored in the diastrophic dysplasia (dtd) mouse. It remains unclear whether bone shortening and deformities are caused solely by changes in the cartilage matrix, or whether chondroitin sulfate proteoglycan undersulfation affects also signalling pathways involved in cell proliferation and differentiation. Therefore we studied macromolecular sulfation in the different zones of the dtd mouse growth plate and these data were related to growth plate histomorphometry and proliferation analysis.A 2-fold increase of non-sulfated disaccharide in dtd animals compared to wild-type littermates in...

Publication year: 2010
Source: Matrix Biology, Volume 29, Issue 4, May 2010, Page 338

[No author name available]

Publication year: 2010
Source: Matrix Biology, Volume 29, Issue 4, May 2010, Page 339

[No author name available]

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 25 April 2010

Amy, Yuen , Carol, Laschinger , Ilana, Talior , Wilson, Lee , Matthew, Chan , ...

Fibrosis is a frequent complication of diabetes mellitus in many organs and tissues but the mechanism of how diabetes-induced glycation of extracellular matrix proteins impacts the formation of fibrotic lesions is not defined. As fibrosis is mediated by myofibroblasts, we investigated the effect of collagen glycation on the conversion of human cardiac fibroblasts to myofibroblasts. Collagen glycation was modeled by the glucose metabolite, methylglyoxal (MGO). Cells cultured on MGO-treated collagen exhibited increased activity of the a-smooth muscle actin promoter and enhanced expression of a-smooth muscle actin, ED-A fibronectin and cadherin, which are markers for myofibroblasts. In cells remodeling floating or...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 21 April 2010

Benito, Casu , Annamaria, Naggi , Giangiacomo, Torri

The heparan sulfate (HS) chains of heparan sulfate proteoglycans (HSPG) are “ubiquitous” components of the cell surface and the extracellular matrix (EC) and play important roles in the physiopathology of developmental and homeostatic processes. Most biological properties of HS are mediated by interactions with “heparin-binding proteins” and can be modulated by exogenous heparin species (unmodified heparin, low molecular weight heparins, shorter heparin oligosaccharides and various nonanticoagulant derivatives of different sizes). Heparin species can promote or inhibit HS activities to different extents depending, among other factors, on how closely their structure mimics the biologically active HS sequences. Heparin shares structural similarities...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 21 April 2010

Benito, Casu , Annamaria, Naggi , Giangiacomo, Torri

The heparan sulfate (HS) chains of heparan sulfate proteoglycans (HSPG) are “ubiquitous” components of the cell surface and the extracellular matrix (EC) and play important roles in the physiopathology of developmental and homeostatic processes. Most biological properties of HS are mediated by interactions with “heparin-binding proteins” and can be modulated by exogenous heparin species (unmodified heparin, low molecular weight heparins, shorter heparin oligosaccharides and various nonanticoagulant derivatives of different sizes). Heparin species can promote or inhibit HS activities to different extents depending, among other factors, on how closely their structure mimics the biologically active HS sequences. Heparin shares structural similarities...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 18 April 2010

Manimalha, Balasubramani , Emanuel M., Schreiber , Joseph, Candiello , G.K., Balasubramani , Justin, Kurtz , ...

Basement membranes (BMs) are physiologically insoluble extracellular matrix sheets present in all multicellular organisms. They play an important role in providing mechanical strength to tissues and regulating cell behavior. Proteomic analysis of BM proteins are challenged by their high molecular weights and extensive post-translational modifications. Here, we describe the direct analysis of an in vivo BM system using a mass spectrometry (MS) based proteomics approach. Retinal BMs were isolated from embryonic chick eyes. The BM macromolecules were deglycosylated and separated by low percentage gradient SDS PAGE, in-gel digested and analyzed by LC-MS/MS. This identified over 27 extracellular matrix proteins in...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 10 April 2010

Jinyoung, Kim , David W., Erikson , Robert C., Burghardt , Thomas E., Spencer , Guoyao, Wu , ...

Attachment and migration of trophectoderm (Tr) cells, hallmarks of blastocyst implantation in mammals, are unique uterine events. Secreted phosphoprotein 1 (SPP1) in the uterus binds integrins on conceptus Tr and uterine luminal epithelium (LE), affecting cell-cell and cell-matrix interactions. The signal transduction pathways activated by SPP1 and integrins in conceptuses have not been elucidated. Results of this study demonstrate that SPP1 binds avß3 and a5ß1 integrins to induce focal adhesion assembly, a prerequisite for adhesion and migration of Tr, through activation of: 1) P70S6K via crosstalk between FRAP1/mTOR and MAPK pathways; 2) mTOR, PI3K, MAPK3/MAPK1 (Erk1/2) and MAPK14 (p38) signaling...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 10 April 2010

Jinyoung, Kim , David W., Erikson , Robert C., Burghardt , Thomas E., Spencer , Guoyao, Wu , ...

Attachment and migration of trophectoderm (Tr) cells, hallmarks of blastocyst implantation in mammals, are unique uterine events. Secreted phosphoprotein 1 (SPP1) in the uterus binds integrins on conceptus Tr and uterine luminal epithelium (LE), affecting cell-cell and cell-matrix interactions. The signal transduction pathways activated by SPP1 and integrins in conceptuses have not been elucidated. Results of this study demonstrate that SPP1 binds avß3 and a5ß1 integrins to induce focal adhesion assembly, a prerequisite for adhesion and migration of Tr, through activation of: 1) P70S6K via crosstalk between FRAP1/mTOR and MAPK pathways; 2) mTOR, PI3K, MAPK3/MAPK1 (Erk1/2) and MAPK14 (p38) signaling...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 9 April 2010

Mahroo K., Parsi , Julian R.J., Adams , John, Whitelock , Mark A., Gibson

Latent transforming growth factor-beta-1 binding protein-2 (LTBP-2) is a protein of poorly understood function associated with fibrillin-1-containing microfibrils during elastinogenesis. In this study we investigated the molecular interactions of LTBP-2 with heparin and heparan sulfate proteoglycans (HSPGs) since unidentified cell surface HSPGs are critical for normal fiber assembly. In solid phase assays, heparin conjugated to albumin (HAC) bound strongly to recombinant full length human LTBP-2. This interaction was completely blocked by addition of excess heparin, but not chondroitin sulfate, confirming specificity. Analysis of binding to LTBP-2 fragments showed that HAC bound strongly to N-terminal fragment LTBP-2 NT(H) and more weakly...

Publication year: 2010
Source: Matrix Biology, Volume 29, Issue 3, April 2010, Page 237

[No author name available]

Publication year: 2010
Source: Matrix Biology, Volume 29, Issue 3, April 2010, Page 238

[No author name available]

Publication year: 2010
Source: Matrix Biology, Volume 29, Issue 3, April 2010, Page 237

[No author name available]

Publication year: 2010
Source: Matrix Biology, Volume 29, Issue 3, April 2010, Page 238

[No author name available]

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 31 March 2010

Gonzalo, Ugarte , Cristian, Santander , Enrique, Brandan

Syndecan-4 and integrins are involved in the cell migration and adhesion processes in several cell types. Syndecan-4, a transmembrane heparan sulfate proteoglycan, is associated to focal adhesions in adherent cells and has been described as a marker of satellite cells in skeletal muscle. In this tissue, ß1 integrin forms heterodimers with a5 and a6 during myoblast differentiation and with a7 in adult muscle. Here, we show that the levels of these two cell surface membrane molecules are regulated by spontaneous electrical activity during the differentiation of rat primary myoblasts. Syndecan-4 and ß1 integrin protein levels decrease after the inhibition of...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 31 March 2010

Joseph, Candiello , Gregory J., Cole , Willi, Halfter

Basement membranes (BMs) are considered to be uniform, approximately 100nm-thin extracellular matrix sheets that serve as a substrate for epithelial cells, endothelial cells and myotubes. To find out whether BMs maintain their ultrastructure, protein composition and biophysical properties throughout life the natural aging history of the human inner limiting membranes (ILM) was investigated. The ILM is a BM at the vitreal surface of the retina that connects the retina with the vitreous. Transmission electron microscopy (TEM) showed that the ILM steadily increases in thickness from 70nm at fetal stages to several microns at age 90. By the age of 20,...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 20 March 2010

Oliver, Gross , Rainer, Girgert , Bogdan, Beirowki , Matthias, Kretzler , Hee Gyung, Kang , ...

Alport syndrome is a hereditary type IV collagen disease leading progressive renal fibrosis, hearing loss and ocular changes. End stage renal failure usually develops during adolescence. COL4A3 -/- mice serve as an animal model for progressive renal scarring in Alport syndrome. The present study evaluates the role of Discoidin Domain Receptor 1 (DDR1) in cell-matrix-interaction involved in pathogenesis of Alport syndrome including renal inflammation and fibrosis.DDR1/COL4A3 double-knockouts were compared to COL4A3 -/- mice with 50% or 100% expression of DDR1, wildtype controls and to DDR1-/- COL4A3 +/+ controls for over 6 years. Double-knockouts lived 47% longer, mice with 50% DDR1...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 20 March 2010

Oliver, Gross , Rainer, Girgert , Bogdan, Beirowki , Matthias, Kretzler , Hee Gyung, Kang , ...

Alport syndrome is a hereditary type IV collagen disease leading progressive renal fibrosis, hearing loss and ocular changes. End stage renal failure usually develops during adolescence. COL4A3 -/- mice serve as an animal model for progressive renal scarring in Alport syndrome. The present study evaluates the role of Discoidin Domain Receptor 1 (DDR1) in cell-matrix-interaction involved in pathogenesis of Alport syndrome including renal inflammation and fibrosis.DDR1/COL4A3 double-knockouts were compared to COL4A3 -/- mice with 50% or 100% expression of DDR1, wildtype controls and to DDR1-/- COL4A3 +/+ controls for over 6 years. Double-knockouts lived 47% longer, mice with 50% DDR1...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 6 March 2010

Pingping, He , Yan, Zhang , Seong Oh, Kim , Ralf J., Radlanski , Kristin, Butcher , ...

Tooth enamel is formed by epithelially-derived cells called ameloblasts, while the pulp dentin complex is formed by the dental mesenchyme. These tissues differentiate with reciprocal signaling interactions to form a mature tooth. In this study we have characterized ameloblast differentiation in human developing incisors, and have further investigated the role of extracellular matrix proteins on ameloblast differentiation. Histological and immunohistochemical analyses showed that in the human tooth, the basement membrane separating the early developing dental epithelium and mesenchyme was lost shortly before dentin deposition was initiated, prior to enamel matrix secretion. Presecretary ameloblasts elongated as they came into contact with...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 1 March 2010

Isabelle, Martelly , Dominique, Singabraya , Aurélie, Vandebrouck , Dulce, Papy-Garcia , Christian, Cognard , ...

Glycosaminoglycans (GAG) are sulfated polysaccharides that play an important role in regulating cell functions. GAG mimetics called RGTAs (for ReGeneraTing Agents) have been shown to stimulate tissue repair. In particular they accelerate myogenesis, in part via their heparin-mimetic property towards growth factors. RGTAs also increase activity of calcium-dependent intracellular protease suggesting an effect on calcium cellular homeostasis. This effect was presently investigated on myoblasts in vitro using one member of the RGTA family molecule named OTR4120. We have shown that OTR4120 or heparin induced transient increases of intracellular calcium concentration ([Ca2+]i) in pre-fusing myoblasts from both mouse SolD7 cell line...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 25 February 2010

Michael A., Chernousov , Kelly, Baylor , Richard C., Stahl , Mark M., Stecker , Lynn Y., Sakai , ...

The extracellular matrix of peripheral nerve is formed from a diverse set of macromolecules, including glycoproteins, collagens and proteoglycans. Recent studies using knockout animal models have demonstrated that individual components of the extracellular matrix play a vital role in peripheral nerve development and regeneration. In this study we identified fibrillin-1 and fibrillin-2, large modular structural glycoproteins, as components of the extracellular matrix of peripheral nerve. Previously it was found that fibrillin-2 null mice display joint contractures, suggesting a possible defect of the peripheral nervous system in these animals. Close examination of the peripheral nerves of fibrillin-2 deficient animals described here...

Publication year: 2010
Source: Matrix Biology, Volume 29, Issue 2, March 2010, Pages 87-88

[No author name available]

Publication year: 2010
Source: Matrix Biology, Volume 29, Issue 2, March 2010, Page 160

[No author name available]

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 14 February 2010

Francesca, Zito , Robert D., Burke , Valeria, Matranga

Pl-nectin is a component of the extracellular matrix that surrounds embryos of the sea urchin Paracentrotus lividus. Pl-nectin mediates adhesion of dissociated embryonic cells to substrates and interfering with ectodermic cells contacting Pl-nectin results in defects in skeleton growth and morphogenesis. Recently, we reported that Pl-nectin is a new member of the discoidin family, in agreement with the notion that many discoidin-containing proteins are involved in cell adhesion processes as integrin ligands. To better understand the molecular basis for the interaction of Pl-nectin with ectoderm, we investigated the hypothesis that Pl-nectin is an integrin ligand in sea urchin embryos. We...

Publication year: 2010
Source: Matrix Biology, In Press, Accepted Manuscript, Available online 12 February 2010

P.W., Kopesky , H.-Y., Lee , E.J., Vanderploeg , J.D., Kisiday , D.D., Frisbie , ...

Our objective was to evaluate the age-dependent mechanical phenotype of bone marrow stromal cell- (BMSC-) and chondrocyte-produced cartilage-like neotissue and to elucidate the matrix-associated mechanisms which generate this phenotype. Cells from both immature (2-4 month-old foals) and skeletally-mature (2-5 year-old adults) mixed-breed horses were isolated from animal-matched bone marrow and cartilage tissue, encapsulated in self-assembling-peptide hydrogels, and cultured with and without TGF-ß1 supplementation. BMSCs and chondrocytes from both donor ages were encapsulated with high viability. BMSCs from both ages produced neo-tissue with higher mechanical stiffness than that produced by either young or adult chondrocytes. Young, but not adult, chondrocytes proliferated...