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Protein Preparation From ...
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PROTEIN ISOLATION/PURIFICATION DISCUSSION BOARD

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SDS removal through dyalisis

chululay 7 117
By chululay
Nov 19, 2008, 8:30 AM

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Immunoprecipitation Optimization

» Pulling down 200+ Kd proteins.
jonathanjacobs 6 822
By DatUVa
Nov 11, 2008, 14:04 PM

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New

Increasing IP Yield

DatUVa 0 42
By DatUVa
Nov 11, 2008, 9:50 AM

Moved

atp sepharose affinity chromatography

» trouble shooting help
Add colour 2 ur life 10 954
By kriodos
Sep 30, 2008, 6:34 AM

Not
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Problematic Basal Expression Levels

wzhang 3 259
By Add colour 2 ur life
Sep 29, 2008, 6:57 AM

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Can I overdry proteins?

Tiffy 1 181
By pguo
Sep 20, 2008, 13:30 PM

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no protein after dialysis

linamira 4 242
By R Bishop
Sep 18, 2008, 10:58 AM

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Trouble seperating tagless protein from Ni-NTA beads

» Protein purification
katiekat 4 291
By kriodos
Sep 04, 2008, 9:21 AM

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Problems with my co-IPs, please help me!!

la biologiste 1 157
By kriodos
Aug 27, 2008, 11:04 AM

Not
New
 

How do you microdialyze a protein (antibody)?

Chemgod1 2 279
By parvoman
Aug 09, 2008, 10:17 AM

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New
 

Identifying and purifying unknown protein

Luke 3 377
By kriodos
Aug 06, 2008, 9:23 AM

Not
New

His-Tag purification - contaminated protein

» additional bands bigger and smaller than my protein
Elayoe 1 481
By kriodos
Jul 21, 2008, 6:40 AM

Moved

His-Tag purification - contaminated protein

» additional bands bigger and smaller than my protein
Elayoe 1 418
By kriodos
Jul 21, 2008, 6:40 AM

Not
New
 

protein degradation after EK cleavage

yang 1 454
By kriodos
Jul 21, 2008, 6:16 AM

Not
New
 

removal of lipids from brain tissue

Satura66 0 156
By Satura66
Jul 21, 2008, 5:44 AM

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New

molar ratio of NTA and His-tagged protein

Aggi 1 338
By R Bishop
Jul 08, 2008, 19:34 PM

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New
 

Protease required!!!!

MKr 2 574
By R Bishop
Jul 02, 2008, 0:55 AM

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New
 

Protein Inhibitors (fast acting)

TRMADR 2 312
By frasermoss
May 09, 2008, 11:45 AM

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New
 

Subcellular fractionation

Tiffy 0 253
By Tiffy
Apr 26, 2008, 11:27 AM

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best leader peptide for periplasmic expression

MKr 1 430
By jcsam
Jan 28, 2008, 21:43 PM
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