WESTERN BLOTTING DISCUSSION BOARD

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Western Blotting Help

exec

2009

By varsha
Nov 21, 2008, 15:03 PM

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Curved bands (frown) in Westerns

BruMom

By qinglongyanyuedao
Nov 21, 2008, 11:01 AM

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Amyloid beta 1-42 and 1-40 detection by Western blot

heela

By mchinmoyee
Nov 20, 2008, 13:50 PM

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Non reducing conditions

jezaxx

2392

By R Bishop
Nov 11, 2008, 9:12 AM

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Strange behavior of lysates

» Problem with Invitrogen pre-cast gels

ShebaAJ

By Murphy
Oct 29, 2008, 11:13 AM

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trouble with coIP assay

contingency

118

By contingency
Oct 22, 2008, 8:44 AM

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No Bands In Western Blotting

wilma

958

By mchinmoyee
Oct 21, 2008, 14:54 PM

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tranfer ok, no bands!!

Pinal Pandya

631

By mchinmoyee
Oct 21, 2008, 14:35 PM

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New

Separating bands on Westerns

RPL

552

By mchinmoyee
Oct 21, 2008, 14:30 PM

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No straight band-WESTERN BLOTTING

user

1642

By mchinmoyee
Oct 21, 2008, 14:23 PM

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New

Playing hide and seek with my protein

Tiffy

147

By R Bishop
Oct 14, 2008, 16:48 PM

Not
New

proteins not detected at predicted MW

» Western blot problems solutions fixes protein dimers glycosylation

Murphy

870

By qinglongyanyuedao
Sep 30, 2008, 9:28 AM

Not
New

Help with Western Blot!

» No signal, ladder is faint.

jxr

601

By Pinal Pandya
Aug 21, 2008, 14:07 PM

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New

Western Blot question

Amtekoth

277

By Amtekoth
Aug 14, 2008, 19:24 PM

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Hot

Protein aggregation ( 1, 2 )

bbarbaro

4795

By R Bishop
Aug 14, 2008, 14:54 PM

Not
New

Problem with western blot detection- strong background and weak signal

Luke

345

By Edward Dougherty
Aug 14, 2008, 14:50 PM

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New

SDS Page on very large (~400kDa) protein

Joe.Sahn

353

By R Bishop
Jul 29, 2008, 17:05 PM

Not
New

Sub-optimal wet transfer buffer?

collmic

410

By R Bishop
Jul 23, 2008, 18:45 PM

Not
New

no detection on purified protein

kema

288

By R Bishop
Jul 02, 2008, 0:59 AM

Not
New

Invitrogen Bis-Tris system

neuromania

290

By R Bishop
May 01, 2008, 11:59 AM

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