Hi, im having loads of difficulties trying to calculate the molar extinction coffient for phosphate at 670nm.
I currently have a stand curve, with the x-axis in micro grams of phosphate and the y-axis with absorbance at 670nm.
could someone explain how to do it i know i have to use the formula e=A/CxL (L is 1cm) but im not sure how to convert the micrograms of phosphate and use it to get the coefficient :-S
if you need any additional information please let me know, i would really appericate if someone could suggest a method to calculate it based on micrograms of phosphate. thanks