I ve been struggling with this problem for weeks!
I tested some primers with a temp gradient PCR and the results were very good from 59° up to 65°
timing 30, 30 , 40 seconds to better test the specificity
but when I swithed to real time pcr (Roche) I found out many unspecific amplifications
even at high temp (65°)
These primers are designed to map a genomic region so I can't really change them much