How to determine the amount of enzyme to use?

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Lavinia Ardianto
Lavinia Ardianto's picture
How to determine the amount of enzyme to use?

Hi everyone,

Currently, I'm working with protease enzyme named Flavourzyme (from Novozyme). It's said that its declared activity = 1000 LAPU/g. 1 LAPU means  the amount of enzyme that hydrolyses 1 µmol of leucine-p-nitroanilide per minute. However I've always seen that mostly the enzyme unit is in U/g (1 Unit of enzyme activity degrades 1 micromole of glycosidic bonds per minute).
Now, I'm getting confused about how to convert enzyme unit from LAPU/g to U/g?? Aren't they just the same?
Then, I want to use the flavourzyme to hydrolyse protein in chicken. I found a reference which also use flavourzyme on chicken and it said:

"The best enzymolysis conditions were temperature 50oC, pH 7, substrate concentration 5%, enzyme dosage 4000 U/g, hydrolysis time 4 hours"
How to prepare enzyme with dosage 4000 U/g?? How much enzyme should I use? Please help me because I'm so confused about it.

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