i am struggling to design my primers for TAF15-201
i am supposed to do it for 2 consecutives exons, but i don't know what i am suppose to do with introns
do i need the full intronic sequence?
how do i link both exonic sequences?
Then once in my primer design tool, and knowing that i need to amplify at least 30bp upstream and downstream of the exons encoding TAF15-201
What are the ranges for my forward and reverse primer what is my pcr size ?
where do my primers sit in my sequence ?
any help would be appreciated