I would like to ask you if someone of you know if it is possible to use mass spectrometry (or something similar) to see the number of glycosylations of a glycoprotein. I am trying to explain a little more.
I work with a protein that has 6 natural N-glycans, with directed-site mutagenesis I introduce an extra N-glycosylation; with western blotting I can see a litlle shift respect to the wild type, but I would like to confirm with another more accurate technique that I have already add this extra N-glycan.
Does anyone have any suggestions? I was thinking about mass spectrometry, just resolve the wild type and the mutant separately and see the difference in their MW, but I am not sure if it is possible.
Thanks in advance.
Detection of Glycosylations-Mass spectrometry