how to prepare 1.5 M Tris HCL buffer (pH- 8.8) ? i am confused between tris base and tris hcl.
Tris-base has very high pH (>10). In order to prepare final pH=8.8, large amount of HCl is required. Thus, one starts with dissolving Tris-base in water, ending up with Tris-HCl after adjusting the pH with HCl to make final pH of 8.8.
It is very simple.simply when you prepare 1.5M Tris buffer it is higly basic nearly 10~11pH .So, to adjust pH 8.8 you have to add HCL till pH doesnot reach 8.8. So, your 8.8 pH Tris buffer contains HCL thats why it is called 1.5M Tris HCL .
I think your confusing is deleted.
thanks a lot.