i want to know the marker for germ cell isolated from testis. ie to find out whether the cells isolated by us is germ cells or not?
It might help in getting an answer to give a little more information about what you are looking for. Questions I have are: What species are you working with? Are you looking for a protein to detect by flow cytometry or microscopy or are you more prone to use PCR to detect gene expression?
Thanks for your reply. I am working on Albino Wistar rats. The rats are about 90days old, adults. i want to isolate germ cells and work on protein expression as well as mrna expression. I am planning to do flowcytometry as well as microscopic studies. If u come across some procedure please send.