I am recently experiencing a difficlty in forming a giga-seal and the whole cell configuration.
(1) I have difficulty in forming a giga-seal (many times only some hundreds MO).
(2) I can rupture the membrane, but the wlole cell has gone within one minute (mostly within some seconds.)
(3) I tried changing the program of lelectrode puller (Narishige) but no improvement. My electrode has 3-5 MO.
(4) Then, I prepare new pipette solution). The 1st patch was excellent (>40 min). Thus, I believed the pipette solution was the cause. But then after in the same day, the same problem came back.
(5) Cells (HEK) seem to be fine.
If you should have the similar experience, would you please give me advises? What should I do?
Thanks in advance.