we are performing siRNA infusion in mouse brain and, usually it works :-)
but this time we have an unexpected effect: the target gene (protein level) is increased in mice treated with the specific siRNA. It means the protein level is higher in the mice treated with the spe-siRNA than in the control siRNA. this control siRNA was already used by us several times and did not cause any adverse problems before. Does any have an explanation?
I thought it could be interferon response, but how will such response target especially our gene of interest?
thanks for any help