I am trying to estimate the amt of Glutathione-S-transferase in the serum of malaria-infected mice.
I used 1mM reduced glutathione+buffer+25microL CDNB(chloro-dinitro benzene)+Post-mitochondrial Supernatant(PMS). I then measured OD at 340nm.
I want to know why we add CDNB in this assay.
CDNB in GST estimation